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Anti-Human CXCL8 Antibody [KT214]

Catalog No. K06289U04D08C
  • Western blotting

    25 ng of recombinant human CXCL8 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT214 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. CXCL8 band was visualized using ECL Western Blotting Substrate. 

    Result: KT214 can detect human CXCL8 by Western blotting.

  • Cross reactivity

    Microtiter wells were coated with various human cytokines. KT214 was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. 

    Result: KT214 does not cross-react with human IL1B, IL2, IL4, IL5, IL6, IL7, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.


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Overview

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  • Product name

    Anti-Human CXCL8 Antibody [KT214]

  • Antibody specificity

    C-X-C motif chemokine ligand 8 (Interleukin 8, IL-8)

  • Species reactivity

    Human

  • Clonality

    Monoclonal

  • Clone number

    KT214

  • Host / isotype

    Mouse / IgG1

  • Immunogen

    Recombinant human CXCL8 (P00726PA)

  • Cross reactivity

    KT214 does not cross-react with human IL1B, IL2, IL4, IL5, IL6, IL7, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF

  • Kinetic characterization by BLI (biolayer interferometry)

    Association rate constant (kon):1.18x 10-5 M-1s-1
    Dissociation rate constant (koff):1.35x 10-3 s-1
    Equilibrium dissociation constant (KD):1.14x 10-8 M

  • Form

    Liquid

  • Concentration

    1 mg/mL in phosphate buffered saline containing 0.09% preservative

  • Conjugation

    Unconjugated

  • Shipping, storage and shelf life

    Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
    Upon receipt,
    * 3 months when stored at 2 to 8 °C
    * 1 year when aliquoted and stored at -20 °C
    * 3 years when aliquoted and stored at -80 °C

Applications

Full details

Recommend concentration

Note

  • Western blot (WB)

    1 µg/mL

  • Indirect ELISA

    1 µg/mL

Note

The applications above have already been verified. The antibody may be suitable for additional applications. 

Optimal antibody concentrations for each application should be determined by the user.

Additional information
  • Target antigen

    • Protein name:

      C-X-C motif chemokine ligand 8

    • Gene name:

      CXCL8

    • Uniprot Accession:

      P10145

    • Organism:

      Homo sapiens (Human)