Anti-Human IL6 Antibody [KT105]
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Sandwich ELISA
Microtiter wells were coated with KT105 at 3 µg/mL as the capture antibody. IL6 was used as the antigen. Peroxidase conjugated mouse anti-human IL6 monoclonal antibody (KT106) was used as the detection antibody.
Result: KT105 and KT106 can be used as a matched antibody pair to detect and quantify the concentration of IL6.
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Full details
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Product name
Anti-Human IL6 Antibody [KT105]
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Antibody specificity
Interleukin-6 (IL-6)
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Species reactivity
Human
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Clonality
Monoclonal
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Clone number
KT105
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Host / isotype
Mouse / IgG2b
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Immunogen
Recombinant human IL-6
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Cross-reactivity
Not tested
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Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon):1.33x 106 M-1s-1
Dissociation rate constant (koff):5.28x 10-4 s-1
Equilibrium dissociation constant (KD): 3.96x 10-10 M -
Purification
Protein A purified from cell culture supernatants
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Form
Liquid
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Concentration
1 mg/mL in phosphate buffered saline containing 0.09% preservative
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Conjugation
Unconjugated
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Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
Upon receipt,
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Full details
Recommend concentration
Note
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Indirect ELISA
1 µg/mL
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Sandwich ELISA
3 µg/mL
KT105 can pair with peroxidase conjugated KT106 for sandwich ELISA. KT105 is used as the capture antibody.
Note
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
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Target antigen
Protein name:
Interleukin 6
Gene name:
IL6
Uniprot Accession:
P05231
Organism:
Homo sapiens (Human)
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Paired antibody information
KT105 may pair with KT106 for sandwich based immune assays.
