25 ng of recombinant human IL1B was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K24005_12D11 at 1 µg/mL was used as the primary antibody and peroxidase-conjugated goat anti-mouse IgG was used as the secondary antibody. IL-1B band was visualized using ECL Western Blotting Substrate.
Result: K24005_12D11 can detect recombinant human IL1B by Western blotting.
Microtiter wells were coated with K24005_12D11 at 3 µg/mL as the capture antibody. Recombinant IL1B (Cat. P00706PA) was used as the antigen. Peroxidase conjugated mouse anti-human IL1B monoclonal antibody (KT216) was used as the detection antibody.
Result: K24005_12D11 and KT216 can be used as a matched antibody pair to detect and quantify the concentration of IL1B.
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Anti-Human IL1B Antibody [K24005_12D11]
Interleukin 1 beta (IL-1 beta)
Host / isotype
Mouse / IgG2b
Recombinant human IL1B (P00706PB)
Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon):2.93x 105 M-1s-1
Dissociation rate constant (koff):3.35x 10-3 s-1
Equilibrium dissociation constant (KD):1.14x 10-8 M
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Protein A purified from cell culture supernatants
Western blot (WB)
K24005_12D11 can pair with peroxidase conjugated KT216 for sandwich ELISA. K24005_12D11 is used as the capture antibody.
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Interleukin 1 beta
Homo sapiens (Human)
Paired antibody information
K24005_12D11 may pair with K24005_1G1 and KT216 for sandwich based immune assays.