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Anti-Human IL1B Antibody [K24005_10C3]

Catalog No. K24005U10C03C
  • Western blotting

    25 ng of recombinant human IL1B was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K24005_10C3 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IL1B band was visualized using ECL Western Blotting Substrate.

    Result: K24005_10C3 can detect recombinant human IL1B by Western blotting.

  • Sandwich ELISA

    Microtiter wells were coated with K24005_10C3 at 3 µg/mL as the capture antibody. Human IL-1B was used as theantigen. Peroxidase conjugated mouse anti-human IL1B monoclonal antibody (KT217) was used as the detection antibody.

    Result: K24005_10C3 and KT217 can be used as a matched antibody pair to detect and quantify the concentration of IL1B. 

  • Cross-reactivity

    Microtiter wells were coated with human cytokines. K24005_10C3 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. 

    Result: K24005_10C3 does not cross-react with human IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.

Associated Products
  • There are no related products


Full details

  • Product name

    Anti-Human IL1B Antibody [K24005_10C3]

  • Antibody specificity

    Interleukin-1 beta (IL-1 beta)

  • Species reactivity


  • Clonality


  • Clone number


  • Host / isotype

    Mouse / IgG2a

  • Immunogen

    Recombinant human IL1B (P00706PB)

  • Cross-reactivity

    K24005_10C3 does not cross-react with human IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.

  • Kinetic characterization by BLI (biolayer interferometry)

    Association rate constant (kon): 1.33x 106 M-1s-1
    Dissociation rate constant (koff):5.63x 10-4 s-1
    Equilibrium dissociation constant (KD): 4.22x 10-10 M

  • Form


  • Concentration

    1 mg/mL in phosphate buffered saline containing 0.09% preservative

  • Conjugation


  • Shipping, storage and shelf life

    Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
    Upon receipt,
    * 3 months when stored at 2 to 8 °C
    * 1 year when aliquoted and stored at -20 °C
    * 3 years when aliquoted and stored at -80 °C

  • Purification

    Protein A purified from cell culture supernatants


Full details

Recommend concentration


  • Western blot (WB)

    1 µg/mL

  • Indirect ELISA

    1 µg/mL

  • Sandwich ELISA

    3 µg/mL

    K24005_10C3 can pair with peroxidase conjugated KT217 for sandwich ELISA. K24005_10C3 is used as the capture antibody.


The applications above have already been verified. The antibody may be suitable for additional applications. 

Optimal antibody concentrations for each application should be determined by the user.

Additional information
  • Target antigen
    • Protein name:

      Interleukin 1 beta

    • Gene name:


    • Uniprot Accession:


    • Organism:

      Homo sapiens (Human)

  • Paired antibody information

    K24005_10C3 may pair with KT216, KT217, K24005_2A2, K24005_2A5, K24005_8D10, K24005_15H5, K24005_5E11 and K24005_7B6 for sandwich based immune assays.