Anti-Human IL1B Antibody [K24005_8D10]
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Western blotting
25 ng of recombinant human IL1B was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K24005_8D10 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IL-1B band was visualized using ECL Western Blotting Substrate.
Result: K24005_8D10 can detect recombinant human IL1B by Western blotting.
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Sandwich ELISA
Microtiter wells were coated with K24005_8D10 at 3 µg/mL as the capture antibody. Recombinant IL1B (Cat. P00706PA) was used as the antigen. Peroxidase conjugated mouse anti-human IL1B monoclonal antibody (K24005_10C3) was used as the detection antibody.
Result: K24005_8D10 and K24005_10C3 can be used as a matched antibody pair to detect and quantify the concentration of IL1B.
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Full details
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Product name
Anti-Human IL1B Antibody [K24005_8D10]
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Antibody specificity
Interleukin-1 beta (IL-1 beta)
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Species reactivity
Human
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Clonality
Monoclonal
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Clone number
K24005_8D10
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Host / isotype
Mouse / IgG2b
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Immunogen
Recombinant human IL1B (P00706PB)
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Cross-reactivity
Not tested
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Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon): 1.29x 106 M-1s-1
Dissociation rate constant (koff):2.95x 10-3 s-1
Equilibrium dissociation constant (KD):2.28x 10-9 M -
Form
Liquid
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Concentration
1 mg/mL in phosphate buffered saline containing 0.09% preservative
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Conjugation
Unconjugated
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Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
Upon receipt,
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C -
Purification
Protein A purified from cell culture supernatants
Full details
Recommend concentration
Note
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Western blot (WB)
1 µg/mL
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Indirect ELISA
1 µg/mL
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Sandwich ELISA
3 µg/mL
K24005_8D10 can pair with peroxidase conjugated K24005_10C3 for sandwich ELISA. K24005_8D10 is used as the capture antibody.
Note
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
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Target antigen
Protein name:
Interleukin 1 beta
Gene name:
IL1B
Uniprot Accession:
P01584
Organism:
Homo sapiens (Human)
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Paired antibody information
K24005_8D10 may pair with K24005_10C3 and K24005_10B3 for sandwich based immune assays.
