25 ng of recombinant human IL1B was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K24005_7B6 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IL1B band was visualized using ECL Western Blotting Substrate.
Result: K24005_7B6 can detect recombinant human IL1B by Western blotting.
Microtiter wells were coated with various human cytokines. K24005_7B6 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Result: K24005_7B6 does not cross-react with human IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.
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Anti-Human IL1B Antibody [K24005_7B6]
Interleukin-1 beta (IL-1 beta)
Host / isotype
Mouse / IgG2a
Recombinant human IL1B (P00706PB)
K24005_7B6 does not cross-react with human IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.
Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon): 9.51x 105 M-1s-1
Dissociation rate constant (koff):1.98x 10-3 s-1
Equilibrium dissociation constant (KD): 2.08x 10-9 M
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Protein A purified from cell culture supernatants
Western blot (WB)
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Interleukin 1 beta
Homo sapiens (Human)