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Anti-Human IL1B Antibody [K24005_7B6]

Catalog No. K24005U07B06C
  • Western blotting

    25 ng of recombinant human IL1B was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K24005_7B6 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IL1B band was visualized using ECL Western Blotting Substrate.

    Result: K24005_7B6 can detect recombinant human IL1B by Western blotting.

  • Cross-reactivity

    Microtiter wells were coated with various human cytokines. K24005_7B6 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. 

    Result: K24005_7B6 does not cross-react with human IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.

Associated Products
  • There are no related products

Overview

Full details

  • Product name

    Anti-Human IL1B Antibody [K24005_7B6]

  • Antibody specificity

    Interleukin-1 beta (IL-1 beta)

  • Species reactivity

    Human

  • Clonality

    Monoclonal

  • Clone number

    K24005_7B6

  • Host / isotype

    Mouse / IgG2a

  • Immunogen

    Recombinant human IL1B (P00706PB)

  • Cross-reactivity

    K24005_7B6 does not cross-react with human IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.

  • Kinetic characterization by BLI (biolayer interferometry)

    Association rate constant (kon): 9.51x 105 M-1s-1
    Dissociation rate constant (koff):1.98x 10-3 s-1
    Equilibrium dissociation constant (KD): 2.08x 10-9 M

  • Form

    Liquid

  • Concentration

    1 mg/mL in phosphate buffered saline containing 0.09% preservative

  • Conjugation

    Unconjugated

  • Shipping, storage and shelf life

    Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
    Upon receipt,
    * 3 months when stored at 2 to 8 °C
    * 1 year when aliquoted and stored at -20 °C
    * 3 years when aliquoted and stored at -80 °C

  • Purification

    Protein A purified from cell culture supernatants

Applications

Full details

Recommend concentration

Note

  • Western blot (WB)

    1 µg/mL

  • Indirect ELISA

    1 µg/mL

Note

The applications above have already been verified. The antibody may be suitable for additional applications. 

Optimal antibody concentrations for each application should be determined by the user.

Additional information
  • Target antigen
    • Protein name:

      Interleukin 1 beta

    • Gene name:

      IL1B

    • Uniprot Accession:

      P01584

    • Organism:

      Homo sapiens (Human)