25ng of recombinant human IL1B was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K24005_1G1 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IL1B band was visualized using ECL Western Blotting Substrate.
Result: K24005_1G1 can detect recombinant human IL1B by Western blotting.
Microtiter wells were coated with K24005_1G1 at 3 µg/mL as the capture antibody. Recombinant human IL1B (Cat. P00706PA) was used as the antigen. Peroxidase conjugated mouse anti-human IL1B monoclonal antibody (K24005_5A2) was used as the detection antibody.
Result: K24005_1G1 and K24005_5A2 can be used as a matched antibody pair to detect and quantify the concentration of IL1B.
Microtiter wells were coated with various human cytokines. K24005_1G1 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Result: K24005_1G1 does not cross-react with human IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.
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Anti-Human IL1B Antibody [K24005_1G1]
Interleukin-1 beta (IL-1 beta)
Host / isotype
Mouse / IgG2b
Recombinant human IL1B (P00706PB)
K24005_1G1 does not cross-react with human IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.
Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon):7.53x 105 M-1s-1
Dissociation rate constant (koff):3.76x 10-4 s-1
Equilibrium dissociation constant (KD):4.99x 10-10 M
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Protein A purified from cell culture supernatants
Western blot (WB)
K24005_1G1 can pair with peroxidase conjugated K24005_5A2 for sandwich ELISA. K24005_1G1 is used as the capture antibody.
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Interleukin 1 beta
Homo sapiens (Human)
Paired antibody information
K24005_1G1 may pair with KT217, K24005_5A2, K24005_5E11, K24005_7B6, K24005_8D10, K24005_10C3, and K24005_12D11 for sandwich based immune assays.