25ng of recombinant human IL1B was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K24005_5E11 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IL1B band was visualized using ECL Western Blotting Substrate.
Result: K24005_5E11 can detect recombinant human IL1B by Western blotting.
Microtiter wells were coated with K24005_5E11 at 3 µg/mL as the capture antibody. Recombinant human IL1B (Cat. P00706PA) was used as the antigen. Peroxidase conjugated mouse anti-human IL1B monoclonal antibody (K24005_10C1) was used as the detection antibody.
Result: K24005_5E11 and K24005_10C1 can be used as a matched antibody pair to detect and quantify the concentration of IL1B.
Microtiter wells were coated with human cytokines. K24005_5E11 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Result: K24005_5E11 does not cross-react with human IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.
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Anti-Human IL1B Antibody [K24005_5E11]
Interleukin-1 beta (IL-1 beta)
Host / isotype
Mouse / IgG2a
Recombinant human IL1B (P00706PB)
K24005_5E11 does not cross-react with human IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, CCL2, KITLG and TNF.
Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon):8.41x 105 M-1s-1
Dissociation rate constant (koff):4.16x 10-4 s-1
Equilibrium dissociation constant (KD): 4.94x 10-10 M
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Protein A purified from cell culture supernatants
Western blot (WB)
K24005_5E11 can pair with peroxidase conjugated K24005_10C1 for sandwich ELISA. K24005_5E11 is used as the capture antibody.
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
Interleukin 1 beta
Homo sapiens (Human)
Paired antibody information
K24005_5E11 may pair with K24005_10C1, K24005_10B3, K24005_1G1, K24005_2A2, K24005_2A5, K24005_13B8, K24005_12D11 for sandwich based immune assays.