Anti-Human IL11 Antibody [KT8]
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Western blotting
25ng of recombinant human IL-11 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT8 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. IL-11 band was visualized using ECL Western Blotting Substrate.
Result: KT8 can detect recombinant human IL-11 by Western blotting.
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Sandwich ELISA
Microtiter wells were coated with KT8 at 5 µg/mL as the capture antibody. Peroxidase conjugated mouse anti-human IL11 monoclonal antibody (KT62) was used as the detection antibody.
Result: KT8 and KT62 can be used as a matched antibody pair to detect and quantify the concentration of IL11.
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Full details
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Product name
Anti-Human IL11 Antibody [KT8]
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Antibody specificity
Interleukin-11 (IL-11)
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Species reactivity
Human
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Clonality
Monoclonal
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Clone number
KT8
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Host / isotype
Mouse / IgG1
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Immunogen
Recombinant human IL-11
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Cross reactivity
Not tested
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Kinetic characterization by BLI (biolayer interferometry)
Not tested
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Form
Liquid
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Concentration
1 mg/mL in phosphate buffered saline containing 0.09% preservative
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Conjugation
Unconjugated
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Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
Upon receipt,
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Full details
Recommend concentration
Note
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Western blot (WB)
1 µg/mL
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Indirect ELISA
1 µg/mL
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Sandwich ELISA
5 µg/mL
KT8 can pair with peroxidase conjugated KT62 for sandwich ELISA. KT8 is used as the capture antibody.
Note
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
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Target antigen
Protein name:
Interleukin 11
Gene name:
IL11
Uniprot Accession:
P20809
Organism:
Homo sapiens (Human)
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Paired antibody information
KT8 may pair with peroxidase conjugated KT62 for sandwich based immune assays.
