25 ng of recombinant human CCL2 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT221 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. CCL2 band was visualized using ECL Western Blotting Substrate.
Result: KT221 can detect recombinant human CCL2 by Western blotting.
Microtiter wells were coated with KT221 at 3 µg/mL as the capture antibody. Human CCL2 was used as the antigen. Peroxidase conjugated mouse anti-human CCL2 monoclonal antibody (K06288_4G5) was used as the detection antibody.
Result: KT221 and K06288_4G5 can be used as a matched antibody pair to detect and quantify the concentration of CCL2.
Microtiter wells were coated with various human cytokines. KT221 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Result: KT221 does not cross-react with human IL1B, IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, KITLG and TNF.
There are no related products
Anti-Human CCL2 Antibody [KT221]
C-C motif chemokine ligand 2 (MCP1)
Host / isotype
Mouse / IgG2b
Recombinant human CCL2 (P00728PA)
KT221 does not cross-react with human IL1B, IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, KITLG and TNF.
Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon): 4.38 x 105 M-1s-1
Dissociation rate constant (koff): 6.51 x 10-5 s-1
Equilibrium dissociation constant (KD): 1.49 x 10-10 M
1 mg/mL in phosphate buffered saline containing 0.09% preservative
Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C
Protein A purified from cell culture supernatants
Western blot (WB)
KT221 can pair with peroxidase conjugated K06288_4G5 for sandwich ELISA. KT221 is used as the capture antibody.
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
C-C motif chemokine ligand 2
Homo sapiens (Human)
Paired antibody information
KT221 may pair with K06288_4G5 and K06288_16E11 for sandwich based immune assays.