Anti-Human CCL2 Antibody [KT221]
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Western blotting
25 ng of recombinant human CCL2 was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT221 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. CCL2 band was visualized using ECL Western Blotting Substrate.
Result: KT221 can detect recombinant human CCL2 by Western blotting.
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Sandwich ELISA
Microtiter wells were coated with KT221 at 3 µg/mL as the capture antibody. Human CCL2 was used as the antigen. Peroxidase conjugated mouse anti-human CCL2 monoclonal antibody (K06288_4G5) was used as the detection antibody.
Result: KT221 and K06288_4G5 can be used as a matched antibody pair to detect and quantify the concentration of CCL2.
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Cross-reactivity
Microtiter wells were coated with various human cytokines. KT221 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Result: KT221 does not cross-react with human IL1B, IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, KITLG and TNF.
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Full details
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Product name
Anti-Human CCL2 Antibody [KT221]
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Antibody specificity
C-C motif chemokine ligand 2 (MCP1)
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Species reactivity
Human
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Clonality
Monoclonal
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Clone number
KT221
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Host / isotype
Mouse / IgG2b
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Immunogen
Recombinant human CCL2 (P00728PA)
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Cross-reactivity
KT221 does not cross-react with human IL1B, IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, CSF2, IFNA, IFNG, KITLG and TNF.
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Kinetic characterization by BLI (biolayer interferometry)
Association rate constant (kon): 4.38 x 105 M-1s-1
Dissociation rate constant (koff): 6.51 x 10-5 s-1
Equilibrium dissociation constant (KD): 1.49 x 10-10 M -
Form
Liquid
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Concentration
1 mg/mL in phosphate buffered saline containing 0.09% preservative
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Conjugation
Unconjugated
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Shipping, storage and shelf life
Shipped at ambient temperature. Avoid repeated freeze-thaw cycles.
Upon receipt,
* 3 months when stored at 2 to 8 °C
* 1 year when aliquoted and stored at -20 °C
* 3 years when aliquoted and stored at -80 °C -
Purification
Protein A purified from cell culture supernatants
Full details
Recommend concentration
Note
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Western blot (WB)
1 µg/mL
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Indirect ELISA
1 µg/mL
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Sandwich ELISA
3 µg/mL
KT221 can pair with peroxidase conjugated K06288_4G5 for sandwich ELISA. KT221 is used as the capture antibody.
Note
The applications above have already been verified. The antibody may be suitable for additional applications.
Optimal antibody concentrations for each application should be determined by the user.
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Target antigen
Protein name:
C-C motif chemokine ligand 2
Gene name:
CCL2
Uniprot Accession:
P13500
Organism:
Homo sapiens (Human)
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Paired antibody information
KT221 may pair with K06288_4G5 and K06288_16E11 for sandwich based immune assays.
