N-15 Labeled Proteins
N-15 labeled proteins can serve as standards for mass spectrometry-based absolute quantification
Protein development
Building the N-15 Labeled Human Proteome
15N-Protein standards for clinical proteomics
Heay isotope-labeled protein standards from Absea
Absolute quantification of protein biomarkers
Standardization for clinical diagnostics
Proof of concept: Profiling of ALD Markers
Absolute protein quantification
>99% N-15 labeling efficiency
- Reduced variability by adding standards at the start of MS sample preparation
- Increased robustness and quantifiable range by having multiple peptides for quantification
References
1.Wahle, M., Remes, P. M., Albrecht, V., Lorenz, M. B., Mueller-Reif, J., Steigerwald, S., ... & Mann, M. (2025). A Novel Hybrid High-Speed Mass Spectrometer Allows Rapid Translation From Biomarker Candidates to Targeted Clinical Tests Using 15N-Labeled Proteins. Molecular & Cellular Proteomics, 24(9).
https://doi.org/10.1016/j.mcpro.2025.101050
2.Cai, X., Geyer, P. E., Perez-Riverol, Y., Omenn, G. S., Dong, L., Winkler, R., ... & Guo, T. (2025). A standardized framework for circulating blood proteomics. Nature Genetics, 1-10.
https://doi.org/10.1038/s41588-025-02319-7