Antibody development (with success-guaranteed option)
Let our team of antibody production experts help you develop a novel polyclonal, monoclonal hybridoma or recombinant antibody. We offer a large portfolio of antibody development solutions covering the entire process from antigen design to antibody purification and screening. Our deep knowledge of antigen determining factors enables us to develop custom antibodies with superior specificity, affinity and assay utility.
Service Details:
Monoclonal antibody production (Hybridoma)
Antigen preparation

optional

Mouse/rat immunization

8-11Weeks

Hybridoma cell preparation and screening

3Weeks

Subcloning

4-6Weeks

Antibody production

2-3Weeks

Sample identification

optional

Monoclonal antibody production (Recombinant)
Antigen preparation

optional

Mouse/rat immunization

8-11Weeks

Hybridoma cell preparation and screening

3Weeks

Antibody sequencing and construction

2-3Weeks

Small scale expression

2Weeks

Antibody production

2-3Weeks

Sample identification

optional

Polyclonal antibody production
Antigen preparation

optional

Immunization

7-8Weeks

Antigen affinity purification

2Weeks

Antibody identification

optional

Large antigen libraries

We are able to prepare a vast diversity of antigens. Our services include DNA synthesis plasmid construction, protein expression, peptide design,peptide synthesis and cross-linking peptides onto carrier proteins.
Various target species

Mouse
Rat
Rabbit
Multiple validation approaches

  • Enzyme-linked Immunosorbent Assay (ELISA)
  • Western blot (WB)
  • Immunohistochemistry (IHC)
  • Chemiluminescent immunoassay (CLIA)
  • Fluorescence Activated Cell Sorting (FACS)
  • Immunoprecipitation (IP)
  • Immunofluorescence (IF)
  • Mass spectrometry (MS)
High product diversity

  • Selection of antibodies for dot blot, western blot and FACS
  • Selection of capture and detection antibody pairs for sandwich ELISA
  • Antibody digestion to Fab or F(ab')2 fragments
  • Antibody labeling
Antibody Customization Service Request Form
To facilitate the successful preparation of your custom antibodies, please provide the necessary information below. Each form should correspond to one antigen only. We will respond to your request promptly.
Required Antibody Type
Rabbit Polyclonal Antibody
Mouse Monoclonal Antibody
Rat Monoclonal Antibody
Requirement for Specific Protein Modification Site Recognition
Yes
No
Antibody Application
Western Blot (WB)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Sandwich ELISA
Flow Cytometry
Other (please specify)
Antibody Isotype Preference
IgG
No Specific Requirement
Source of Antigen
Provided by Customer
Purchased from Absea Product Catalog
Customized by Absea
Additional Services Required from Absea
Antibody Purification
Western Blot Analysis
Immunoprecipitation Analysis
Flow Cytometry Analysis
Neutralizing Antibody Screening
Specificity Antibody Screening
Sandwich ELISA Antibody
Antibody Sequencing
Affinity Testing (via Bio-Layer Interferometry)
Antibody Labeling (HRP, Biotin, FITC)
Preparation of Antibody Fragments (Fab fragments, F(ab’)2fragments)
Submit