Antibody development
Let our team of antibody production experts help you develop a novel polyclonal, monoclonal hybridoma or recombinant antibody. We offer a large portfolio of antibody development solutions covering the entire process from antigen design to antibody purification and screening. Our deep knowledge of antigen determining factors enables us to develop custom antibodies with superior specificity, affinity and assay utility.
Service Details:
Monoclonal antibody production (Hybridoma)
Antigen preparation

optional

Mouse/rat immunization

8-11Weeks

Hybridoma cell preparation and screening

3Weeks

Subcloning

4-6Weeks

Antibody production

2-3Weeks

Sample identification

optional

Monoclonal antibody production (Recombinant)
Antigen preparation

optional

Mouse/rat immunization

8-11 weeks

Hybridoma cell preparation and screening

3 weeks

Antibody sequencing and construction

2-3 weeks

Small scale expression

4-6 weeks

Antibody production

2-3 weeks

Sample identification

optional

Polyclonal antibody production
Antigen preparation

optional

Immunization

7-10Weeks

Antigen affinity purification

2Weeks

Antibody identification

optional

Large antigen libraries

We are able to prepare a vast diversity of antigens. Our services include DNA synthesis plasmid construction, protein expression, peptide design,peptide synthesis and cross-linking peptides onto carrier proteins.
Various target species

Mouse
Rat
Rabbit
Multiple validation approaches

  • Enzyme-linked Immunosorbent Assay (ELISA)
  • Western blot (WB)
  • Immunohistochemistry (IHC)
  • Chemiluminescent immunoassay (CLIA)
  • Fluorescence Activated Cell Sorting (FACS)
  • Immunoprecipitation (IP)
  • Immunofluorescence (IF)
  • Mass spectrometry (MS)
High product diversity

  • Selection of antibodies for dot blot, western blot and FACS
  • Selection of capture and detection antibody pairs for sandwich ELISA
  • Antibody digestion to Fab or F(ab')2 fragments
  • Antibody labeling