Antibody development
Let our team of antibody production experts help you develop a novel polyclonal, monoclonal hybridoma or recombinant antibody. We offer a large portfolio of antibody development solutions covering the entire process from antigen design to antibody purification and screening. Our deep knowledge of antigen determining factors enables us to develop custom antibodies with superior specificity, affinity and assay utility.
Service Details:
Monoclonal antibody production (Hybridoma)
Antigen preparation
optional
Mouse/rat immunization
8-11Weeks
Hybridoma cell preparation and screening
3Weeks
Subcloning
4-6Weeks
Antibody production
2-3Weeks
Sample identification
optional
Monoclonal antibody production (Recombinant)
Antigen preparation
optional
Mouse/rat immunization
8-11 weeks
Hybridoma cell preparation and screening
3 weeks
Antibody sequencing and construction
2-3 weeks
Small scale expression
4-6 weeks
Antibody production
2-3 weeks
Sample identification
optional
Polyclonal antibody production
Antigen preparation
optional
Immunization
7-10Weeks
Antigen affinity purification
2Weeks
Antibody identification
optional
Large antigen libraries
We are able to prepare a vast diversity of antigens. Our services include DNA synthesis plasmid construction, protein expression, peptide design,peptide synthesis and cross-linking peptides onto carrier proteins.
Various target species
Mouse
Rat
Rabbit
Rat
Rabbit
Multiple validation approaches
- Enzyme-linked Immunosorbent Assay (ELISA)
- Western blot (WB)
- Immunohistochemistry (IHC)
- Chemiluminescent immunoassay (CLIA)
- Fluorescence Activated Cell Sorting (FACS)
- Immunoprecipitation (IP)
- Immunofluorescence (IF)
- Mass spectrometry (MS)
High product diversity
- Selection of antibodies for dot blot, western blot and FACS
- Selection of capture and detection antibody pairs for sandwich ELISA
- Antibody digestion to Fab or F(ab')2 fragments
- Antibody labeling